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高效液相色谱法测定跌打活络微乳中蛇床子素的含量
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【免费医学论文网】作者：王利胜 占建坤 周莉玲【关键词】 高效液相色谱摘要：目的建立跌打活络微乳中蛇床子素含量的测定 方法 。方法采用高效液相色谱法，色谱柱：Diamonsil钻石C18（5 μm，250 mm×4.6 mm）；流动相：甲醇水（80∶20），检测波长：322 nm；流速：1 ml/min。结果蛇床子素在0.042~ 0.336 μg线性关系良好，r=0.999 7，平均回收率为100.91%，RSD为2.42%(n=6)。结论该法操作简便，结果准确，灵敏度高，重现性好，可用于该制剂的质量控制。
关键词：跌打活络微乳；高效液相色谱法；蛇床子素
Determination of Osthole in Dieda Huoluo Microemulsions by HPLC
Abstract：ObjectiveTo establish the determination method for osthole in Dieda Huoluo Microemulsions. MethodsHPLC was used with DiamonsilC18 column (250 mm×4.6 mm, 5 μm), detection wavelength at 322 nm, mobile phase: methanol-water (80∶20), flow rate: 1ml/min. ResultsOsthole has good linear relationship between 0.042~0.336 μg, r=0.999 7, the recovery of osthole was 100.91% and RSD was 2.42% (n=6). Conclusion The method is simple, sensitive and reliable to carry out to control the quality of this preparation.
Key words：Dieda Hu
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