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富锗灵芝菌丝体氨基酸及超氧化物歧化酶含量的测定
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【制药工程毕业论文】作者：党建章, 孙海燕， 许柏球， 冯丽雄【关键词】 灵芝；,,富锗；,,氨基酸；,,超氧化物歧化酶摘要：目的：测定富锗灵芝菌丝体氨基酸及超氧化物歧化酶(SOD)的含量，探讨培养基中GeO2的最适加量。 方法 ：高效液相色谱仪 分析 氨基酸，氯化硝基四氮唑蓝（NBT）光化还原法测定SOD的含量。结果：培养基中添加400 mg・L-1的GeO2，灵芝菌丝体中氨基酸含量比对照提高54.34%，SOD提高16.73%。结论：为富锗灵芝菌丝体的 应用 奠定基础。
关键词：灵芝； 富锗； 氨基酸； 超氧化物歧化酶
Determination of SOD and Amino Acids in Geenriched Mycelia of Ganoderma lucidum
Abstract:Objective:To determine the contents of SOD and amino acids in Geenriched mycelia of Ganoderma lucidum and explore the optimum dosage of GeO2 in culture medium.Methods: The contents of amino acids were analyzed by high performance liquid chromatography and SOD was determined by nitroblue tetrazolium photoreduction method.Results:Adding GeO2 400 mg・L-1 to the culture medium the contents of amino acids and SOD in Geenriched mycelia of Ganoderma lucidum increase by 54.34% and 16.73% respectively over normal mycel
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