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高效液相色谱法测定栽培射干中次野鸢尾黄素的含量
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【##西医 论文】【摘要】 目的 应用 高效液相色谱法测定栽培射干中次野鸢尾黄素的含量，考察其药材的内在质量。 方法 采用高效液相色谱法，色谱柱为TSK ge1 ODS 80 TS250 mm×4.6 mm；流动相为甲醇磷酸水溶液（pH=3）∶53%∶47%；流速为1 ml/min；检测波长为266 nm；柱温35℃。结果次野鸢尾黄素在0.0118～0.118 μg范围内与色谱峰面积呈线性关系（r=0.999 9）；平均加样回收率为99.84%，RSD为2.059%，次野鸢尾黄素的含量为0.1252%。结论该法简便、准确、灵敏度高，栽培射干中的次野鸢尾黄素的含量大于0.10%，符合《 中国 药典》要求。
【关键词】 高效液相色谱法 射干 次野鸢尾黄素
Abstract：ObjectiveTo determine irisflorentin in cultivated Belamcanda chinensis(L.)DC by HPLC,and to inspect the quality of its ingredients.MethodsUsing HPLC with TSK ge1 ODS Column80(250 mm×4.6 mm),the mobile phase was composed of a mixture of methanol-phosphoric aicd aqueous soluton (pH=3)∶53%∶47%,the flow rate was 1 ml/min,the UV detection wavelength was set at 265 nm,and the temperature was at 35℃.ResultsIrisflorentin showed linear relationship with peak areas in the range of 0.0118～0.118 μg(r=0.999 9). The average recovery was 99.84%,RSD was 2.
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